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cd74 antibody  (Bioss)


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    Bioss cd74 antibody
    Cd74 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd74 antibody/product/Bioss
    Average 92 stars, based on 6 article reviews
    cd74 antibody - by Bioz Stars, 2026-02
    92/100 stars

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    Cd74 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibodies cd74 antibody  (Bioss)
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    anti-cd74 polyclonal antibody thermofisher pa5-22113  (Thermo Fisher)
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    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
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    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
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    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
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    fluorescein isothiocyanate fitc conjugate  (Bioss)
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    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
    Fluorescein Isothiocyanate Fitc Conjugate, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate fitc conjugate/product/Bioss
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    fluorescein isothiocyanate fitc conjugate - by Bioz Stars, 2026-02
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    cd74 polyclonal antibody kit  (Bioss)
    92
    Bioss cd74 polyclonal antibody kit
    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
    Cd74 Polyclonal Antibody Kit, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd74 polyclonal antibody kit - by Bioz Stars, 2026-02
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    cd74 polyclonal antibody kit fluorescein isothiocyanate (fitc) conjugate  (Bioss)
    90
    Bioss cd74 polyclonal antibody kit fluorescein isothiocyanate (fitc) conjugate
    <t>CD74</t> is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.
    Cd74 Polyclonal Antibody Kit Fluorescein Isothiocyanate (Fitc) Conjugate, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    cd74 polyclonal antibody kit fluorescein isothiocyanate (fitc) conjugate - by Bioz Stars, 2026-02
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    CD74 is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.

    Journal: World Journal of Stem Cells

    Article Title: Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

    doi: 10.4252/wjsc.v16.i12.1022

    Figure Lengend Snippet: CD74 is a target of microRNA-540-3p and regulates P65 expression. A: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of microRNA-540-3p (miR-540-3p) targets; B: A dual-luciferase assay was used to detect the binding of miR-540-3p to CD74; C: Co-IP was used to detect the binding of CD74 and nuclear factor-kappaB (NF-κB) in dendritic cells (DCs); D: Co-IP was used to detect the binding of CD74 and NF-κB in T cells; E-G: Western blot analysis of the expression of CD74 and NF-κB in DCs; H-J: Western blot analysis of the expression of CD74 and NF-κB in T cells; K-M: Western blot analysis of CD74 and NF-κB expression in DCs after co-culture with T cells; N-P: Western blot analysis of the expression of CD74 and NF-κB in T cells after co-culture with DCs; Q-S: Western blot analysis of the expression of CD74 and NF-κB in DCs after plasmid transfection; T-V: Western blot analysis of CD74 and NF-κB expression in T cells after plasmid transfection. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. Mut: Mutant; BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; exo: Exosomes; NF-κB: Nuclear factor-kappaB.

    Article Snippet: After blocking with 5% bovine serum albumin (Solarbio Co., Ltd, Beijing, China), the membrane was incubated overnight at 4 °C with anti-CD74 polyclonal antibody (1:1000 dilution; ThermoFisher PA5-22113), anti-NFκB p65 monoclonal antibody (1:200 dilution; ThermoFisher 436700), and anti-GAPDH monoclonal antibody (1:1000 dilution; Abmart M20006).

    Techniques: Expressing, Luciferase, Binding Assay, Co-Immunoprecipitation Assay, Western Blot, Co-Culture Assay, Plasmid Preparation, Transfection, Mutagenesis

    Exosome-derived miR-540-3p alleviates immune resistance after heterotopic heart transplantation. A: Histopathological observation of the heart after heterotopic heart transplantation using hematoxylin and eosin staining; B: Detection and calculation of the difference in ejection fraction before and after heterotopic heart transplantation; C: Detection and calculation of differences in the percentage of fractional shortening before and after heterotopic heart transplantation; D-G: Enzyme-linked immunosorbent assay-based quantitative analysis of cytokine production (interleukin-1β, interferon-γ, interleukin-10, transforming growth factor β1) in serum; H: Expression of microRNA-540-3p in extracted dendritic cells (DCs); I: Expression of microRNA-540-3p in extracted T cells; J-M: Flow cytometric analysis of the positive rate of cell surface markers in DCs (major histocompatibility complex II, CD80, CD86, and CD274); N-P: Flow cytometric analysis of the positive rate of T cells (CD4 + T, CD8 + T, and T regulatory cells); Q-S: Western blot analysis of the expression of CD74 and nuclear factor-kappaB (NF-κB) in extracted DCs; T-V: Western blot analysis of CD74 and NF-κB expression in the extracted T cells; W: Co-IP was used to detect the binding of CD74 and NF-κB in extracted DCs; X: Co-IP was used to detect the binding of CD74 and NF-κB in extracted T cells. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; NF-κB: Nuclear factor-kappaB; MHC: Major histocompatibility complex; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon.

    Journal: World Journal of Stem Cells

    Article Title: Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

    doi: 10.4252/wjsc.v16.i12.1022

    Figure Lengend Snippet: Exosome-derived miR-540-3p alleviates immune resistance after heterotopic heart transplantation. A: Histopathological observation of the heart after heterotopic heart transplantation using hematoxylin and eosin staining; B: Detection and calculation of the difference in ejection fraction before and after heterotopic heart transplantation; C: Detection and calculation of differences in the percentage of fractional shortening before and after heterotopic heart transplantation; D-G: Enzyme-linked immunosorbent assay-based quantitative analysis of cytokine production (interleukin-1β, interferon-γ, interleukin-10, transforming growth factor β1) in serum; H: Expression of microRNA-540-3p in extracted dendritic cells (DCs); I: Expression of microRNA-540-3p in extracted T cells; J-M: Flow cytometric analysis of the positive rate of cell surface markers in DCs (major histocompatibility complex II, CD80, CD86, and CD274); N-P: Flow cytometric analysis of the positive rate of T cells (CD4 + T, CD8 + T, and T regulatory cells); Q-S: Western blot analysis of the expression of CD74 and nuclear factor-kappaB (NF-κB) in extracted DCs; T-V: Western blot analysis of CD74 and NF-κB expression in the extracted T cells; W: Co-IP was used to detect the binding of CD74 and NF-κB in extracted DCs; X: Co-IP was used to detect the binding of CD74 and NF-κB in extracted T cells. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; DC: Dendritic cell; LPS: Lipopolysaccharide; NF-κB: Nuclear factor-kappaB; MHC: Major histocompatibility complex; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon.

    Article Snippet: After blocking with 5% bovine serum albumin (Solarbio Co., Ltd, Beijing, China), the membrane was incubated overnight at 4 °C with anti-CD74 polyclonal antibody (1:1000 dilution; ThermoFisher PA5-22113), anti-NFκB p65 monoclonal antibody (1:200 dilution; ThermoFisher 436700), and anti-GAPDH monoclonal antibody (1:1000 dilution; Abmart M20006).

    Techniques: Derivative Assay, Transplantation Assay, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Immunopeptidomics, Western Blot, Co-Immunoprecipitation Assay, Binding Assay

    Exosomes derived from microRNA-540-3p overexpressing bone marrow mesenchymal stem cells promote immune tolerance to cardiac allograft via the CD74/nuclear factor-kappaB pathway. BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon; NF-κB: Nuclear factor-kappaB.

    Journal: World Journal of Stem Cells

    Article Title: Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft

    doi: 10.4252/wjsc.v16.i12.1022

    Figure Lengend Snippet: Exosomes derived from microRNA-540-3p overexpressing bone marrow mesenchymal stem cells promote immune tolerance to cardiac allograft via the CD74/nuclear factor-kappaB pathway. BMSC: Bone marrow mesenchymal stem cell; IDO: Indoleamine 2,3-dioxygenase; IL: Interleukin; TGF: Transforming growth factor; IFN: Interferon; NF-κB: Nuclear factor-kappaB.

    Article Snippet: After blocking with 5% bovine serum albumin (Solarbio Co., Ltd, Beijing, China), the membrane was incubated overnight at 4 °C with anti-CD74 polyclonal antibody (1:1000 dilution; ThermoFisher PA5-22113), anti-NFκB p65 monoclonal antibody (1:200 dilution; ThermoFisher 436700), and anti-GAPDH monoclonal antibody (1:1000 dilution; Abmart M20006).

    Techniques: Derivative Assay